Coding

Part:BBa_K4275031:Design

Designed by: Maggie Li   Group: iGEM22_GreatBay_SCIE   (2022-10-10)


PfuFerritin-wt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. The amino acid sequence of Pfuferritin is derived from the genomic sequence of Pyrococcus furiosus whilst its DNA sequence is codon-optimized based on the codon-usage table of E.coli Strain K12.MG1655.

Source

Pyrococcus furiosus

References

1. "Fan, Kelong et al. “Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension.” Analytical chemistry vol. 90,9 (2018): 5671-5677. doi:10.1021/acs.analchem.7b05217

2. Li, Thomas L et al. “Engineering a Genetically Encoded Magnetic Protein Crystal.” Nano letters vol. 19,10 (2019): 6955-6963. doi:10.1021/acs.nanolett.9b02266

3. Tatur, Jana et al. “A highly thermostable ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus.” Extremophiles : life under extreme conditions vol. 10,2 (2006): 139-48. doi:10.1007/s00792-005-0484-x

4. Aubry, Mary et al. “Engineering E. coli for Magnetic Control and the Spatial Localization of Functions.” ACS synthetic biology vol. 9,11 (2020): 3030-3041. doi:10.1021/acssynbio.0c00286"