Part:BBa_K4275031:Design
PfuFerritin-wt
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. The amino acid sequence of Pfuferritin is derived from the genomic sequence of Pyrococcus furiosus whilst its DNA sequence is codon-optimized based on the codon-usage table of E.coli Strain K12.MG1655.
Source
Pyrococcus furiosus
References
1. "Fan, Kelong et al. “Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension.” Analytical chemistry vol. 90,9 (2018): 5671-5677. doi:10.1021/acs.analchem.7b05217
2. Li, Thomas L et al. “Engineering a Genetically Encoded Magnetic Protein Crystal.” Nano letters vol. 19,10 (2019): 6955-6963. doi:10.1021/acs.nanolett.9b02266
3. Tatur, Jana et al. “A highly thermostable ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus.” Extremophiles : life under extreme conditions vol. 10,2 (2006): 139-48. doi:10.1007/s00792-005-0484-x
4. Aubry, Mary et al. “Engineering E. coli for Magnetic Control and the Spatial Localization of Functions.” ACS synthetic biology vol. 9,11 (2020): 3030-3041. doi:10.1021/acssynbio.0c00286"